Conjugated Protein Domains as Engineered Scaffold Proteins

Guanylate kinase domains of the MAGUK family scaffold proteins as specific phospho-protein-binding modules.

Membrane-associated guanylate kinases (MAGUKs) are a large family of scaffold proteins that play essential roles in tissue developments, cell-cell communications, cell polarity control, and cellular signal transductions. Despite extensive studies over the past two decades, the functions of the signature guanylate kinase domain (GK) of MAGUKs are poorly understood. Here we show that the GK domai...

Scaffold proteins as dynamic switches.

often assembled into their supramolecular complexes and targeted to specific regions of cells by multimodular scaffold proteins. The success of this organization and localization is critical for signaling efficiency and specificity. The catalytically inactive scaffold proteins that perform these tasks are traditionally viewed as passive molecules that " glue " signaling proteins together. Howev...

Fiber recruiting peptides: noncovalent decoration of an engineered protein scaffold.

Fiber Recruiting (FiRe) peptides are described. These are derivatives of self-assembling fiber (SAF) forming peptides that are conjugated with small molecules (in our case, biotin or a FLAG-tag octapeptide). The FiRe peptides are co-assembled into fibers and used as bait to recruit folded and functional proteins to the fiber surfaces. This was demonstrated using two molecular recognition models...

Decellularized Cartilage as a Scaffold for Tissue Engineered Cartilage

Discovering Domains Mediating Protein Interactions

Background: Protein-protein interactions do not provide any direct information re‌garding the domains within the proteins that mediate the interactions. The majority of proteins are multi domain proteins and the interaction between them is often defined by the pairs of their domains. Most of the former studies focus only on interacting do‌main pairs. However they do not consider the in...